Loss of heterozygosity of chromosome 20 in sporadic colorectal cancer.
- Author:
Zhihai PENG
1
;
Chongzhi ZHOU
;
Fang ZHANG
;
Yun LING
;
Huamei TANG
;
Shaochun BAI
;
Wanqing LIU
;
Guoqiang QIU
;
Lin HE
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Aged; Aged, 80 and over; Chromosomes, Human, Pair 20; Colorectal Neoplasms; genetics; pathology; Female; Humans; Loss of Heterozygosity; Male; Microsatellite Repeats; Middle Aged
- From: Chinese Medical Journal 2002;115(10):1529-1532
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo analyze the loss of heterozygosity (LOH) of chromosome 20 in patients with sporadic colorectal cancer to identify additional loci involved in colorectal tumorigenesis.
METHODSPolymorphic microsatellite markers were analyzed in 83 colorectal cancer patients' tumor and normal DNA by PCR. PCR products were electrophoresed on an 377 DNA sequencer. Genescan 2.1 and Genotype 2.1 software were used in the LOH scanning and analysis. Comparisons between LOH frequency and clinicopathological data were performed by chi(2) test. P < 0.05 was considered statistically significant.
RESULTSThe average LOH frequency in the long arm, short arm and whole chromosome 20 was 21.1%, 26.7% and 22.8%, respectively. Chromosome 20 exhibited relatively high LOH frequency, particularly in the regions of 20p and 20q11.1-q13.1.
CONCLUSIONThere is notable genetic instability on chromosome 20 in sporadic colorectal carcinoma patients; that is, mutation on chromosome 20 is closely associated with sporadic colorectal carcinogenesis. Also, there may be tumor suppressor genes related to sporadic colorectal carcinoma near the region 20q11.1-q13.1.
