Study of isolation of fluoroquinolone-resistant Ureaplasma urealyticum and identification of mutant sites.
- Author:
Wenbo ZHANG
1
;
Yimou WU
;
Weiguo YIN
;
Minjun YU
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Substitution; Anti-Infective Agents; pharmacology; DNA Gyrase; genetics; DNA Topoisomerase IV; genetics; Drug Resistance, Multiple, Bacterial; genetics; Fluoroquinolones; Humans; Mutation; Polymerase Chain Reaction; Ureaplasma urealyticum; drug effects; genetics; isolation & purification
- From: Chinese Medical Journal 2002;115(10):1573-1575
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo study the resistance mechanism of clinical isolates of Ureaplasma urealyticum resistant to fluoroquinolones.
METHODSThirteen isolates of Ureaplasma urealyticum resistant to six fluoroquinolones were selected out of 184 clinical isolates and their QRDRs (quinolone resistance-determining region) gyrA, gyrB, parC and parE were amplified by PCR. Sequencing results were compared to those susceptible reference strains and a comparison of deduced amino acid sequences were performed.
RESULTSSequence comparison revealed a C to A change at 87nt of gyrA QRDR leading to the substitution of Asp95 with glutamic acid and a C to T change at 50nt of parC QRDR leading to the substitution of Ser80 with leucine.
CONCLUSIONThese results suggest that a C to A change at 87nt of gyrA QRDR and a C to T change at 50nt of parC QRDR are associated with fluoroquinolone resistance of Ureaplasma urealyticum.
