The cross-reactivity of I-A(g7) and I-A(d) tetramers.
- Author:
Yong PENG
1
;
Chih-pin LIU
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Animals; Cross Reactions; Female; Glutamate Decarboxylase; immunology; Histocompatibility Antigens Class II; immunology; Mice; Mice, Inbred BALB C; Mice, Inbred NOD; Molecular Sequence Data; T-Lymphocytes; immunology
- From: Chinese Medical Journal 2002;115(10):1579-1581
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the cross-reactivity between glutamic acid decarboxylase (GAD)-I-A(g7) and I-A(d) tetramer in diabetes-prone non-obese diabetic (NOD) mice (I-A(g7)) and diabetes-free Balb/c mice (I-A(d)).
METHODSTwo GAD peptide I-A(g7) and I-A(d) tetramers were generated and compared for phenotype and function of sorted GAD peptide I-A(g7) and I-A(d) tetramer-positive (tet+) T cells.
RESULTSThe cross-reactivity is shown in either tetramer positive percentage or tetramer staining intensity. The NOD and Balb/c derived-tet+ T cells were able to be cross-stained by GAD peptide I-A(g7) and I-A(d) tetramers, and responded to both irradiated NOD and Balb/c splenotyes under stimulation by synthetic and recombinant GAD peptides.
CONCLUSIONAlthough I-A(g7) and I-A(d) are closely related in biochemical and biological aspects, their most notable difference is the presence or absence of a negatively charged residue at position beta57 that links to insulin-dependent diabetes mellitus.
