pcDNAL1 genetic immunization can induce specific cell-mediated immune responses in C57BL/6 mice.
- Author:
Jianming SONG
1
;
Xiangle SUN
;
Tianju LIU
;
Yili WANG
;
Lüsheng SI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Hypersensitivity, Delayed; etiology; Immunization; Interferon-gamma; biosynthesis; Lymphocyte Activation; Mice; Mice, Inbred C57BL; Papillomaviridae; immunology; Spleen; cytology; Vaccines, DNA; immunology; Viral Vaccines; immunology
- From: Chinese Medical Journal 2002;115(11):1697-1700
- CountryChina
- Language:English
-
Abstract:
OBJECTIVETo investigate the specific cell-mediated immune efficacy of the an HPV16 prophylactic vaccine.
METHODSC57BL/6 mice were randomly divided into 3 groups: experimental group I (treated with pcDNA L1), control group II (treated with pcDNA3.1) and control group III (treated with PBS buffer). The mice were immunized three times during a three-week interval. Ten to fourteen days after the third inoculation, a footpad swelling test was used to detect delayed-type hypersensitivity (DTH) responses. Antigen-specific splenocyte proliferation assay and quantitation of IFN-gamma cells in splenocytes were performed by FACS assay.
RESULTSIn the experimental group, splenocytes actively proliferated after stimulation with HPV16 VLP, and had developed a markedly larger amount of CD8(+) IFN-gamma(+) cells, which is an index for special CTL. Also, the footpad was significantly thickened upon inoculation with HPV16 VLP.
CONCLUSIONNaked DNA vaccine of HPV16 L1 can induce specific cell-mediated immune responses in mice, which should be considered for evaluation of HPV16 DNA vaccine feasibility.
