Generation and identification of P210(T315I-BCR/ABL) transgenic mice.
- VernacularTitle:P210T315I-BCR/ABL转基因小鼠模型的建立与鉴定
- Author:
Yufeng ZHU
1
;
Yuanzhan WANG
1
;
Fanyi MENG
1
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Fusion Proteins, bcr-abl; Genetic Vectors; Genotype; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; Mice; Mice, Transgenic; Promoter Regions, Genetic
- From: Chinese Journal of Hematology 2015;36(3):221-224
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct the P210(T315I-BCR/ABL) transgenic mice model.
METHODSThe transgenic vector in which the P210(T315I-BCR/ABL) gene and eGFP gene was derived by APN/CD13 promoter was constructed and microinjected into the single-cell fertilized eggs of C57 mice. Transgene integration was conformed by PCR genotyping and P210(T315I-BCR/ABL) expression levels was evaluated by RT-PCR. The CML phenotype was confirmed by blood routine examination, Wright's staining for peripheral blood and bone marrow smears, HE staining for organs of transgenic mice.
RESULTSThree transgenic mice lines with high expression of P210(T315I-BCR/ABL) gene and eGFP gene was selected. Compared with the wild type mice, the levels of WBC, platelet and neutrophil granulocyte of transgenic mice began to increase gradually at 2 months, and increase to 23.9×10⁹/L, 4 136×10⁹/L, and 74.6% respectively at 6 months. The remarkable hyperplasia of granulocytes was seen in the peripheral blood and bone marrow smears with splenomegaly infiltrated by leukemic cells.
CONCLUSIONThe P210(T315I-BCR/ABL) transgenic mice was constructed and provided a model to explore the mechanism of T315I CML and screen out the drug for T315 CML patient.
