Mechanism of downregulation of apoptosis by autophagy induced by oridonin in HeLa cells.
- Author:
Qiao CUI
1
;
Shin-ichi TASHIRO
;
Satoshi ONODERA
;
Takashi IKEJIMA
Author Information
1. China-Japan Research Institute of Medical Pharmaceutical Sciences, Shenyang Pharmaceutical University, Shenyang 110016, China.
- Publication Type:Journal Article
- MeSH:
Antineoplastic Agents, Phytogenic;
isolation & purification;
pharmacology;
Apoptosis;
drug effects;
Autophagy;
drug effects;
Blotting, Western;
Cytochromes c;
metabolism;
Diterpenes;
isolation & purification;
pharmacology;
Diterpenes, Kaurane;
isolation & purification;
pharmacology;
Flow Cytometry;
HeLa Cells;
Humans;
Isodon;
chemistry;
Plant Leaves;
chemistry;
Plants, Medicinal;
chemistry;
Proto-Oncogene Proteins c-bcl-2;
metabolism;
Sirtuin 1;
Sirtuins;
metabolism;
bcl-2-Associated X Protein;
metabolism
- From:
Acta Pharmaceutica Sinica
2007;42(1):35-39
- CountryChina
- Language:Chinese
-
Abstract:
To study the mechanism of downregulation of apoptosis by autophagy induced by oridonin in HeLa cells, the cell viability was measured by MTT method. DNA fragmentation was assayed by agarose gel electrophoresis. Autophagic and apoptotic ratio was determined by flowcytometric analysis. Protein expression was detected by Western blotting analysis. Oridonin induced both apoptosis and autophagy in HeLa cells. Apoptosis was upregulated by introduction of the inhibitor of autophagy, 3-methyladenine (3-MA). Addition of oridonin increased Bax/Bcl-2 expression ratio and cytochrome c, whereas the expression of SIRT-1 was decreased, and 3-MA pre-application enhanced these changes. Oridonin-induced autophagy antagonized apoptosis in HeLa cells through mitochondrial pathway.
