Pharmacokinetics of pingyangmycin hydrochloride in rabbits determined by microdialysis coupled with RP-HPLC.
- Author:
Jian SHI
1
;
Zi-Bin GAO
;
Jing WEI
;
Ping-Tian DING
;
Da-Wei CHEN
Author Information
1. The Forth Hospital of Hebei Medical University, Shijiazhuang 050011, China.
- Publication Type:Journal Article
- MeSH:
Animals;
Antibiotics, Antineoplastic;
administration & dosage;
blood;
pharmacokinetics;
Area Under Curve;
Bleomycin;
analogs & derivatives;
blood;
chemistry;
pharmacokinetics;
Chromatography, High Pressure Liquid;
methods;
Female;
Injections, Intravenous;
Male;
Microdialysis;
methods;
Molecular Structure;
Rabbits
- From:
Acta Pharmaceutica Sinica
2007;42(3):297-300
- CountryChina
- Language:Chinese
-
Abstract:
Microdialysis coupled with RP-HPLC was used to study the blood pharmacokinetics of pingyangmycin hydrochloride in rabbits. Supelco RP-amide C16 column was adopted for the analysis of pingyangmycin hydrochloride. The data was analyzed with 3P87 program. The calibration curve was linear in the concentration range from 1.04 to 66.56 microg x mL(-1) (r2 = 0.999 4). The in vivo recovery of microdialysis probe was (42.8 +/- 3.4)% (n = 4). The concentration-time curve of pingyangmycin hydrochloride was fitted to two-compartment model. T1/2 alpha and T1/2 beta were 14.9 and 60.3 min, respectively. The method is proved to be accurate, simple and suitable for the pharmacokinetics study of pingyangmycin hydrochloride in rabbits.
