Phosphorylated modification of genistein and their interaction with lysozyme.
- Author:
Xiao-lan CHEN
1
;
Zhang-qi YU
;
Ling-bo QU
;
Jin-wei YUAN
;
Jian-sha LU
;
Yu-fen ZHAO
Author Information
1. Key Laboratory of Chemical Biology, Department of Chemistry, Zhengzhou University, Zhengzhou 450052, China. chenxl@zzu.edu.cn
- Publication Type:Journal Article
- MeSH:
Drug Interactions;
Genistein;
analogs & derivatives;
chemical synthesis;
metabolism;
Magnetic Resonance Spectroscopy;
Muramidase;
chemistry;
Phosphorylation;
Protein Interaction Mapping;
methods;
Spectrometry, Mass, Electrospray Ionization
- From:
Acta Pharmaceutica Sinica
2007;42(4):396-400
- CountryChina
- Language:Chinese
-
Abstract:
A series of genistein's phosphates were synthesized through a simplified Atherton-Todd reaction and the structures of the phosphates were determined by electrospray ionization mass spectrometry (ESI-MS) and NMR. In case of monophosporyl derivatives, NMR spectra show that substitutions occur at the 7-position of genistein but not at its 4' and 5-position. Moreover, the non-covalent complexes of lysozyme with the four new genistein phosphates were detected by ESI-MS.
