In vitro anti-angiogenic action of lambda-carrageenan oligosaccharides.
- Author:
Hai-Min CHEN
1
;
Xiao-Jun YAN
;
Feng WANG
;
Jing LIN
;
Wei-Feng XU
Author Information
1. Key Laboratory of Marine Biotechnology, Ningbo University, Ningbo 315211, China. chenhaimin@nbu.edu.cn
- Publication Type:Journal Article
- MeSH:
Angiogenesis Inhibitors;
pharmacology;
Animals;
Carrageenan;
pharmacology;
Cell Movement;
drug effects;
Cell Proliferation;
drug effects;
Chick Embryo;
Chorioallantoic Membrane;
blood supply;
cytology;
drug effects;
Endothelial Cells;
drug effects;
Humans;
Matrix Metalloproteinase 2;
analysis;
Oligosaccharides;
pharmacology
- From:
Acta Pharmaceutica Sinica
2007;42(6):595-600
- CountryChina
- Language:Chinese
-
Abstract:
This study was designed to evaluate the inhibition effect of lambda-carrageenan oligosaccharides on neovascularization in vitro by chick chorioallantoic membrane (CAM) model and human umbilical vein endothelial cell ( HUVEC). lambda-Carrageenan oligosaccharides caused a dose-dependent decrease of the vascular density of CAM, and adversely affected capillary plexus formation. At a high concentration of 1 mg x mL(-1), this compound inhibited the endothelial cell proliferation, while low concentration of lambda-carrageenan oligosaccharides (< 250 microg x mL(-1)) affected the cell survival slightly (> 95%). Different cytotoxic sensitivity of lambda-carrageenan oligosaccharides in three kinds of cells was observed, of which HUVEC is the most sensitive to this oligosaccharides. The inhibitory action of lambda-carrageenan oligosaccharides on the endothelial cell invasion and migration was also observed at relatively low concentration (150 - 300 microg x mL(-1)) through down-regulation of intracellular matrix metalloproteinases-2 (MMP-2) expression on endothelial cells. Current observations demonstrated that lambda-carrageenan oligosaccharides are potential angiogenesis inhibitor with combined effects of inhibiting invasion, migration and proliferation.
