Inhibition of duck hepatitis B virus DNA replication by antisense phosphorothioate oligodeoxynucleotides in vitro and in vivo.
- Author:
Biao DONG
1
;
Xing-wu SHAO
;
Pei-zhen TAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; DNA Replication; drug effects; DNA, Viral; drug effects; Ducks; Hepadnaviridae Infections; virology; Hepatitis B Surface Antigens; blood; Hepatitis B Virus, Duck; genetics; physiology; Hepatitis, Viral, Animal; virology; Oligodeoxyribonucleotides, Antisense; pharmacology; Protein Precursors; blood; Virus Replication; drug effects
- From: Chinese Journal of Experimental and Clinical Virology 2003;17(1):25-27
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo determine the feasibility of inhibition of duck hepatitis B virus (DHBV) DNA replication by antisense phosphorothioate oligodeoxynucleotides corresponding to DHBV transcription region.
METHODSThe authors designed three antisense phosphorothioate oligodeoxynucleotides which correspond to DHBV PreS1,PreS2 and S antigen gene promotors respectively. The DNA replication level was detected with Southern blot method and cpm calculation.
RESULTSPrimary duck hepatocyte culture was treated with 1.5 micromol/L antisense oligodeoxynucleotides in vitro, all the antisense fragments caused a firm inhibition of viral DNA replication and the inhibition rates were 61.5%, 69.3% and 62.4%, respectively. In vivo, the animals were treated with 10 microgram/g PreS1 antigen gene promotor antisense oligodeoxynucleotides per day for 6 days and a very strong inhibition rate of 87.9% was obtained.
CONCLUSIONSThe results demonstrated the potential clinical application of antisense phosphorothioate oligodeoxynucleotides in clinics.
