Sequence analysis of the deletion and mutation in carboxy terminal region of the Epstein-Barr virus latent membrane protein 1 derived from nasopharyngeal carcinoma patients.
- Author:
Min-zhong TANG
1
;
Yu-ming ZHENG
;
Xiu-chan GUO
;
Yong-li ZHANG
;
Yi ZENG
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Base Sequence; Gene Deletion; Herpesvirus 4, Human; genetics; Humans; Molecular Sequence Data; Nasopharyngeal Neoplasms; virology; Point Mutation; Sequence Analysis, DNA; Viral Matrix Proteins; genetics
- From: Chinese Journal of Experimental and Clinical Virology 2003;17(1):35-38
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo study the deletion and mutation in carboxy terminal region of LMP1 gene derived from nasopharyngeal carcinoma (NPC) in Guangdong and Guangxi, the high risk areas of nasopharyngeal carcinoma in China.
METHODSLMP1 gene carboxy terminal region was amplified from nasopharyngeal carcinoma tissues by PCR, and then cloned and sequenced.
RESULTSOf the 20 cases, 17 were LMP1 positive. In all positive cases, only 1 case did not show deletion. Four positive cases were chosen for DNA sequencing, The rusult showed that all the four cases had mutation and the 30bp deletion.
CONCLUSIONSHigh frequency of deletion and mutation in LMP1 gene of nasopharyngeal carcinoma tissues was found in Guangdong and Guangxi. Whether it related to the high incidence of NPC should be further studied.
