Activating effect of hepatitis B virus preS/S protein on proliferating cell nuclear antigen gene promoter.
- Author:
Pei-jun YAN
1
;
Lei WANG
;
Xi-liang ZHA
;
Chang-de LU
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Hepatocellular; genetics; virology; Hepatitis B Surface Antigens; genetics; Hepatitis B virus; genetics; Humans; Liver Neoplasms; genetics; virology; Proliferating Cell Nuclear Antigen; genetics; Promoter Regions, Genetic; Protein Precursors; genetics; Tumor Cells, Cultured; Virus Integration
- From: Chinese Journal of Experimental and Clinical Virology 2003;17(1):42-45
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDPreS/S gene was derived from hepatitis B virus (HBV) integration fragment of human hepatocellular carcinoma genome, containing the promoter of preS/S and the C terminal truncated preS/S open reading frame. PreS/S protein may have important roles in processing hepatoma in some HBV-infected patients. The aim of the study was to study the activity of HBV preS/S protein on proliferating cell nuclear antigen (PCNA) promoter and to localize compartment of the preS/S protein in the liver cell line L02.
METHODSThe authors studied the effect of the 3 -truncated preS/S on human PCNA promoter by co-transfecting the expression plasmids of luciferase reporter gene, used the immunohistochemical method to localize the preS/S protein.
RESULTSThe expression product of the plasmid, pKSH7C-HpaI which contained the 3 -truncated preS/S and the flanking cellular sequences, stimulated the expression of PCNA promoter dose dependently,and its effect was 0.5 folds higher than control. Immunohistochemistry showed that the preS/S protein located in the cytosolic region of the liver cell line L02.
CONCLUSIONSThe HBV preS/S protein could stimulate the PCNA promoter of the liver cell, its effect was not direct, which suggests that the effect of preS/S protein on PCNA promoter was probably through the cell signal transduction pathway.
