In vitro infection of human liver cancer cell line HepG2 with HCV.
- Author:
Zhi-qiang SONG
1
;
Fei HAO
;
Qiao-yu MA
;
Yu-ming WANG
Author Information
- Publication Type:Journal Article
- MeSH: Carcinoma, Hepatocellular; pathology; virology; Coculture Techniques; Hepacivirus; genetics; growth & development; physiology; Hepatitis C, Chronic; virology; Humans; Liver Neoplasms; pathology; virology; RNA, Viral; analysis; biosynthesis; genetics; Tumor Cells, Cultured; Viral Nonstructural Proteins; biosynthesis; genetics; Virus Replication
- From: Chinese Journal of Experimental and Clinical Virology 2003;17(1):77-80
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo test susceptibility of human liver cell line Hep G2 to HCV in vitro.
METHODSHep G2 was cultivated with the serum from a chronic hepatitis C patient. After inoculation, plus and minus strand of HCV RNA, the expression of HCV NS3 antigen and the location of HCV RNA in cell and/or supernatant were examined by RT-PCR, immunohistochemistry and in situ hybridization, respectively.
RESULTSHCV RNA could be detected from day 2 to day 40 post-inoculation in both cell and supernatant. HCV NS3 antigen could be expressed in infected cells and HCV RNA was mainly situated within cytoplasm.
CONCLUSIONSThe results suggested that HepG2 cell line was not only susceptible to HCV but also could support its replication in vitro.
