Development of recombinant human-derived IgG antibody against HBsAg.
- Author:
Yu GUO
1
;
Mi-fang LIANG
;
Sheng-li BI
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antibodies, Monoclonal; biosynthesis; immunology; Antigen-Antibody Reactions; Cell Line; Hepatitis B Antibodies; biosynthesis; immunology; Hepatitis B Surface Antigens; immunology; Humans; Immunoglobulin Fab Fragments; genetics; Immunoglobulin G; biosynthesis; immunology; Peptide Library; Recombinant Proteins; biosynthesis; immunology; Spodoptera
- From: Chinese Journal of Experimental and Clinical Virology 2004;18(2):105-108
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo investigate preparation of human-derived anti-HBs IgG antibody.
METHODSmRNA was extracted from lymphocytes of a volunteer with high level antibody to HbsAg. Fab phage display library was constructed. Purified HBsAg was used to screen the Fab. Eukaryotic expression vector was constructed and transfected into sf9 cells, then the supernatant was purified. The expression of anti-HBs human IgG was tested with blocking ELISA and immunofluorescent test.
RESULTSA whole human anti-HBs IgG molecule was obtained, the antibody reacts with HBsAg "a" determinant.
CONCLUSIONIt can be concluded preliminarily that this antibody has neutralizing characteristic against HbsAg.
