Construction of replication-deficient recombinant adenovirus expressing gag-polDelta and gp140TM genes of human immunodeficiency virus in mice.
- Author:
Ying LIU
1
;
Lan WU
;
Ke-ming ZHOU
;
Xu-dong ZHANG
;
Kun-sue HONG
;
Yi-ming SHAO
Author Information
- Publication Type:Journal Article
- MeSH: AIDS Vaccines; immunology; Adenoviridae; genetics; Animals; Female; Fusion Proteins, gag-pol; biosynthesis; genetics; Gene Products, env; biosynthesis; genetics; HIV-1; genetics; immunology; Mice; Mice, Inbred BALB C; Recombination, Genetic; Transfection; Vaccines, DNA; immunology; env Gene Products, Human Immunodeficiency Virus
- From: Chinese Journal of Experimental and Clinical Virology 2004;18(2):150-153
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDConstruction of replication-deficient recombinant adenovirus expressing gag-pol and env genes of human immunodeficiency virus (HIV) in mice.
METHODSgag-polDelta and gp140TM genes were cloned into shuttle vector pAdTrack-CMV respectively, and then the plasmids containing gag-polDelta or gp140TM gene were cotransformed with the backbone of adenovirus into E.coli BJ5183. Transfections of the recombinants were performed to obtain recombinant adenoviruses. Its immunogenicity was evaluated by testing antibody levels of mice primed with DNA vaccines and boosted with recombinant adenoviruses.
RESULTSThe replication-deficient recombinant adenovirus could express Gp140TM, Gag P55 and P24 proteins correctly. The mice primed with DNA vaccines and boosted with recombinant adenoviruses elicited high titer of HIV-1-specific antibody compared with that inoculated with DNA vaccines only.
CONCLUSIONReplication-deficient recombinant adenovirus expressing gag-polDelta and gp140TM can elicit high titer HIV-1-specific antibodies.
