Significance of detecting HBV-DNA by the fluorescence quantitative PCR.
- Author:
Bao-mei WAN
1
;
Shu-jun LIU
;
Xin-jun LIU
;
Yan-ming YIN
;
He-lou LI
;
Yong-xing TAN
Author Information
- Publication Type:Journal Article
- MeSH: Adolescent; Adult; Aged; Child; DNA, Viral; blood; Female; Fluorescence; Hepatitis B; virology; Hepatitis B virus; genetics; physiology; Humans; Male; Middle Aged; Polymerase Chain Reaction; methods; Virus Replication
- From: Chinese Journal of Experimental and Clinical Virology 2004;18(2):162-164
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo study the correlativity between HBV-DNA and the markers of hepatitis B virus infection and different clinical types of hepatitis B.
METHODSThe fluorescence quantitation (FQ) of HBV-DNA of 105 patients with hepatitis B was performed by PCR, and the correlativity between the fluorescence quantitation of HBV-DNA and the markers of hepatitis B virus and different clinical types of hepatitis B was analyzed.
RESULTSNinety-seven percent of the patients were found HBsAg(+), HBeAg(+), HBcAb(+); 75% were HBsAg(+), HBeAb(+), HBcAb(+); 60% were HBsAg(+), HBcAb(+); 40% were HBsAg(+); in HBsAb(+), HBeAb(+), HBcAb(+) (or both HBsAb and HBcAb were positive) group the HBV DNA was undetectable. The analysis indicated that there was a significant difference among different groups (P less than 0.05).HBV-DNA was detected in 72.2% in acute hepatitis B group, in 75% of chronic hepatitis B group, and in 70% of cases of liver cirrhosis with hepatitis B group. The analysis indicated that there was no significant difference among the different clinical types of hepatitis (P greater than 0.05).
CONCLUSIONThe levels of viral replication were not correlated with different clinical types of hepatitis B; the concentration of HBV-DNA in serum was related to hepatitis B antigen.
