Study on clinical isolates of human cytomegalovirus found in Urumqi by restriction fragment length polymorphism of PCR products.
- Author:
Xue-lei YANG
1
;
Na-er JIA
;
He SUN
;
Wen-hui DU
Author Information
- Publication Type:Journal Article
- MeSH: China; epidemiology; Cytomegalovirus; genetics; isolation & purification; Cytomegalovirus Infections; epidemiology; virology; DNA-Directed DNA Polymerase; genetics; Humans; Immediate-Early Proteins; genetics; Polymerase Chain Reaction; methods; Polymorphism, Restriction Fragment Length; Viral Envelope Proteins; genetics
- From: Chinese Journal of Experimental and Clinical Virology 2004;18(2):168-171
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDThe study was designed to investigate the status of molecular epidemiology of HCMV in Urumqi through genetic comparison of clinical isolates.
METHODSDNA sequences of 2.0-2.6 kb were amplified by polymerase chain reaction from three relatively conservative gene regions (DNA polymerase, glycoproteins H, and major immediate-early antigen) of 28 clinical HCMV strains and then were analysed by restriction enzymes.
RESULTSThe restriction patterns of the clinical isolates which did not have relation in epidemiology were greatly different, but the patterns of the clinical isolates related in epidemiology such as strains paired in mother and infant were quite similar. Of eight mother and infant pairs, from whom HCMV were isolated, four pairs showed identity of restriction profiles within each pair for all three amplified regions, four pairs showed differences between mother and infant.
CONCLUSIONThese results confirm the high degree of genetic variability among cytomegalovirus strains in Urumqi. Analysis of PCR-RFLP can indicate transmission of HCMV infection and facilitate its molecular epidemiologic studies.
