The construction of attenuated Tiantan recombinant vaccinia virus vector with IFN-gamma receptor gene deletion.
- Author:
Wei HUANG
1
;
Ying LIU
;
Dan-li DUAN
;
Hai-shan LI
;
Yong LIU
;
Kun-Xue HONG
;
Jia-hong ZHU
;
Yi-ming SHAO
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cell Line; Chick Embryo; Gene Deletion; Genetic Vectors; Humans; Rabbits; Receptors, Interferon; genetics; physiology; Recombination, Genetic; Vaccines, Attenuated; immunology; Vaccinia virus; genetics; immunology; pathogenicity; Virulence; Virus Replication
- From: Chinese Journal of Experimental and Clinical Virology 2004;18(1):43-46
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVEB8R gene encodes a secreted protein with homology to IFN-gamma receptor, which neutralizes the antiviral and immunological regulation activities of IFN-gamma. To improve the safety of vaccinia virus vector, an attenuated recombinant vaccinia virus with the B8R gene deletion from Tiantan vaccine strain (VTT) was constructed.
METHODSThe transfer vectors were generated by joining B8R left flank, B8R right flank, vv promoter, LacZ, multicloning site and pBRSK fragments. The recombinant viruses VTTdeltaB8RLacZ (VTT with B8R deletion and LacZ insertion) were constructed by homologous recombination.
RESULTSThe B8R deletion mutants were confirmed by dot blot with B8R gene probe and PCR amplification. The replication ability of VTTdeltaB8RLacZ strain in vitro was similar to that of the VTT. The skin lesions formed by VTTdeltaB8RLacZ (10(6) pfu) were significantly smaller and healed faster than those formed by VTT when injected intradermally to the rabbits,and no visible ulceration occurred. Meanwhile LacZ in VTKgpedeltaB8RLacZ was expressed stably.
CONCLUSIONSThe attenuated vector with B8R gene deletion improves the safety of recombinant vaccinia virus vaccine B8R locus may be used as a new site for insertion of foreign genes in vaccinia virus vector.
