Capacity of HIV enzyme immunoassay diagnostic kits to detect antibodies against different genotypes of HIV.
- Author:
Ai-jing SONG
1
;
Xiu-hua LI
;
Juan LI
;
Si-hong XU
;
Feng ZHANG
;
Yan-min WAN
;
Chun-tao ZHANG
;
You-chun WANG
Author Information
- Publication Type:Journal Article
- MeSH: Enzyme-Linked Immunosorbent Assay; Genotype; HIV; genetics; HIV Antibodies; blood; Humans; RNA, Viral; blood; Reagent Kits, Diagnostic; Reverse Transcriptase Polymerase Chain Reaction; Sensitivity and Specificity
- From: Chinese Journal of Experimental and Clinical Virology 2003;17(4):319-321
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the capacity of commercial HIV enzyme immunoassay (EIA) diagnostic kits to detect antibodies against different genotypes of HIV.
METHODSHIV RNA was detected with RT-PCR from samples positive for HIV antibody. The purified PCR products were sequenced directly and the genotypes of HIV from samples were analyzed. The samples for each genotype of HIV were diluted and the diluted samples were detected with different HIV EIA diagnostic kits.
RESULTSAll 20 samples positive for HIV antibody were also positive for HIV RNA; 9 of 20 isolates were genotype B, 9 of them were genotype C or CRF BC, 2 of them were CRF AE. The sensitivity of different HIV EIA diagnostic kits to detect antibodies against different genotypes of HIV was not significantly different.
CONCLUSIONThe capacity of commercial HIV diagnostic kits to detect antibodies against different HIV genotypes may not be significantly different.
