Nucleotides sequence analysis of EV71 Chinese strain SHZH98 3C segment.
- Author:
Ya-qing HE
1
;
Fan YANG
;
Liang-cheng LI
;
Qi JIN
Author Information
- Publication Type:Journal Article
- MeSH: Amino Acid Sequence; Base Sequence; Enterovirus; genetics; isolation & purification; Genes, Viral; Humans; Molecular Sequence Data; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Amino Acid; Sequence Homology, Nucleic Acid
- From: Chinese Journal of Experimental and Clinical Virology 2003;17(4):354-357
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the EV71 Chinese strain SHZH98 and analyze its genetic evolution using 3c gene as index.
METHODSThe 3C gene cDNA of EV71 Chinese strain SHZH98 was amplified by PCR, the PCR product was sequenced.
RESULTSThe EV71 Chinese mainland strain SHZH98 3C segment was 549 bps in length. Comparison of nucleotide sequences from other enteroviruses which have been published, revealed a higher homology to strain MS, 78.7% at nucleotide level and 93.45% at deduced amino acid level. The homology to strain BrCr was 76.7% at nucleotide level and 89.1% at deduced amino acid level. Taiwan strains POLY,NCKU,TW2086,TW2272 shared a lower homology with Chinese mainland strain SHZH98, 74.0%, 73.8%, 71.9%, 69.8% at nucleotide level and 90.7%, 90.2%, 84.2%, 82.5% at deduced amino acid level. The genetic progress analysis revealed that EV71 Chinese mainland strain SHZH98 3C segment shares more homology with European and American strains than Taiwan strains.
CONCLUSIONThe non-structural protein of EV71 Chinese strains may have different evolutionary process from Taiwan strains.
