Establishing DNA chip technique for detecting hepatitis C virus genotypes and primary application.
- Author:
Hong-ju MAO
1
;
Shi-min GU
;
Jian-long ZHAO
;
Jiang-xia LIU
Author Information
- Publication Type:Journal Article
- MeSH: 5' Untranslated Regions; genetics; Genotype; Hepacivirus; classification; genetics; Humans; Nucleic Acid Hybridization; Oligonucleotide Array Sequence Analysis; methods; Polymerase Chain Reaction; RNA, Viral; blood; Sensitivity and Specificity
- From: Chinese Journal of Experimental and Clinical Virology 2003;17(4):375-377
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a new method based DNA chip technique for detecting HCV genotypes.
METHODSGenotyping probes were designed according to the sequence of HCV 5' NCR to generate DNA chip. The probes on DNA chip contains 5 major genotypes and 8 subtypes. The DNA fragment amplified by labeling Cy5 fluorescence was hybridized with DNA chip.
RESULTSFifty-five out of 65 isolates detected by DNA chip belonged to 1b- DNA sequencing of form a part of the isolates was used as the control. The results of both were completely consistent.
CONCLUSIONThe method is simple and rapid with high specificity and sensitivity. It can be applied in detection of HCV RNA and genotypes.
