Development of the cDNA chip for SARS virus and a primary study on the possible molecular mechanism of interferon alpha2b inhibiting the SARS virus replication.
- Author:
Yue-long SHU
1
;
Zhao-jun DUAN
;
Zheng WANG
;
Mei-sheng SUN
;
Jing ZHANG
;
Li-lan ZHANG
;
Xue-jun MA
;
Jun-ping PENG
;
Qi JIN
;
Yun-de HOU
Author Information
- Publication Type:Journal Article
- MeSH: Humans; Interferon-alpha; pharmacology; Oligonucleotide Array Sequence Analysis; methods; RNA, Viral; genetics; Recombinant Proteins; SARS Virus; drug effects; genetics; isolation & purification; physiology; Severe Acute Respiratory Syndrome; virology; Virus Replication; drug effects
- From: Chinese Journal of Experimental and Clinical Virology 2003;17(3):209-212
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUNDTo study the molecular mechanism of interferon alpha2b(IFNalpha2b) inhibiting the SARS virus replication. SARS-associated coronavirus (SARS virus) cDNA chip was developed and applied to detect the virus RNA transcription levels in the interferon-treated and untreated cell cultures, and the mechanism of anti-SARS virus activity of interferon alpha2b in cell culture system was explored.
METHODSSARS virus cDNA chip was prepared by comparing the published SARS virus genome sequence, and the cDNA chip was used to study the interferon alpha2b function during SARS virus replication.
RESULTSSARS virus cDNA chip was successfully prepared by using PCR method. The results showed that the cDNA chip could be used to detect the viral RNA transcription level. Interferon alpha2b could inhibit almost all the SARS virus gene transcription. An unknown gene at the position 28130-28426 bp, named as U gene, may play an important role during the viral replication.
CONCLUSIONA SARS virus whole genome cDNA chip was established. It could be used to study the virus molecular biology and antiviral drug screening. The results also showed that interferon alpha2b could inhibit almost the whole virus gene transcription by using the cDNA chip.
