Callus induction of Cinnamonum camphora and formation of borneol.
- Author:
Meilan CHEN
1
;
Zhengliang YE
;
Shaolin OUYANG
;
Shufang LIN
;
Aijuan SHAO
;
Luqi HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Bornanes; metabolism; Cinnamomum camphora; growth & development; metabolism; Culture Media; Tissue Culture Techniques
- From: China Journal of Chinese Materia Medica 2010;35(5):558-560
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo optimize the condition of callus of Cinnamonum camphora induced.
METHODGC and plant tissue culture method were applied in the study.
RESULTThe effect of callus induced and the growth of callus were different in MS medium with different proportion of hormone. The ration of callus induced was the highest and the growth of callus was the most prosperous in the MS medium with 4 mg x L(-1) 2,4-D and 0.2 mg x L(-1) 6-BA. It is found that callus induced by young leaf contained borneol, but callus induced by young stem not.
CONCLUSIONThe optimization of callus of C. camphora induced is using the MS medium with 4 mg x L(-1) 2,4-D + 0.2 mg x L(-1) 6-BA. Callus induced by young leaf can generate borneol.
