HPLC determination of six components in zedoary turmeric oil and its related injections.
- Author:
Huan HE
1
;
Shuangcheng MA
;
Songjiu TIAN
;
Qiming ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Curcuma; chemistry; Injections; Oils, Volatile; analysis
- From: China Journal of Chinese Materia Medica 2010;35(5):593-597
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an HPLC method to simultaneously determine six ingredients in zedoary turmeric oil and its related injections.
METHODHPLC analysis was performed on Waters Symmetry C18 (4.6 mm x 250 mm, 5 microm). The mobile phase was composed of methanol and water solution in gradient elution mode with at a flow rate of 1.0 mL x min(-1). The column temperature was 30 degrees C and the UV detection wavelength was 215 nm.
RESULTThe six ingredients were separated well. The linear ranges of curdione, curcumol, germacrone, curzerene, furanodiene and beta-elemene were 1.74-347.00, 2.38-475.00, 2.49-497.00, 2.07-826.00, 5.05-1009.00, 4.78-955.00 mg x L(-1) (r > or = 0.9996), respectively. The average recoveries were above 95% (RSD <3.0%, n=9).
CONCLUSIONThe method is accurate, reliable and reproducible, it can be used for the quality control of zedoary turmeric oil and its related injections.
