Quantitative analysis of aglycone of momordicoside L from Momordica charantia in different areas by HPLC.
- Author:
Yu ZHANG
1
;
Jian GUAN
;
Jiongmo CUI
;
Yuqing ZHAO
Author Information
- Publication Type:Journal Article
- MeSH: Calibration; Chromatography, High Pressure Liquid; methods; Momordica charantia; chemistry; Saponins; analysis
- From: China Journal of Chinese Materia Medica 2010;35(5):620-622
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC for determination of aglycone of momordicoside L in Momordica charantia.
METHODA Kromasil C18 (4.6 mm x 150 mm, 5 microm) column was used. The mobile phase was acetonitrile-H2O (64:36), the flow rate was 1.0 mL x min(-1) and the UV detection wavelength was set 203 nm.
RESULTThe calibration curves were linear from of 0.025 microg to 1 microg (r =0.9911), the contents of aglycone of momordicoside L in Shandong, Henan, Hebei, Jiangxi are 0.211, 0.033, 0.013, 0.007 mg x g(-1), respectively.
CONCLUSIONThe method is simple and reliable for determination of aglycone of momordicoside L in M. charantia.
