In vitro activity of baicalin against non-albicans Candida biofilms.
- Author:
Changzhong WANG
1
;
Huijuan CHENG
;
Xiongfei ZHANG
;
Shengli XU
;
Yan GUAN
;
Lihua YU
;
Yun YUN
Author Information
- Publication Type:Journal Article
- MeSH: Biofilms; drug effects; Candida; drug effects; physiology; Flavonoids; pharmacology; Microbial Sensitivity Tests
- From: China Journal of Chinese Materia Medica 2010;35(5):639-641
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of baicalin against Candida glabrata, C. parapsilosis, C. krusei and C. guilliermondii biofilms.
METHOD96-well microtitre plates were used to set up the biofilms; microdilution method was applied to detect minimal inhibitory concentration (MIC) of baicalin for the four non-albians Candida, and XTT reduction assay was adopted to determine sessile minimal inhibitory concentration (SMIC) of baicalin against the four isolates and to detect the effects on adhesion of the fungal cells.
RESULTMICs of baicalin for the four non-albians Candida cells were 125, 250, 125, 62.5 mg L(-1), respectively. The four non-albians Candida could form mature biofilms on 96-well microtitre plates. SMIC50 of baicalin for the four isolates were > 1000, 500, 125, 250 mg x L(-1), respectively. SMIC80 for the four isolates were greater than or equal to 1000 mg x L(-1). Baicalin showed potent inhibitory effects on adhesion of the four non-albians Candida cells.
CONCLUSIONBaicalin displays substantial inhibitory effects on C. parapsilosis, C. krusei and C. guilliermondii biofilm.
