Effects of light on flavonoids accumulation and related gene expression in suspension cultures of Scutellaria baicalensis.
- Author:
Shunqin CHEN
1
;
Yuan YUAN
;
Yujiang LUO
;
Luqi HUANG
;
Ping CHEN
;
Xiaoming LI
Author Information
- Publication Type:Journal Article
- MeSH: Antioxidants; pharmacology; Cell Culture Techniques; Chromatography, High Pressure Liquid; Drugs, Chinese Herbal; pharmacology; Flavanones; analysis; genetics; radiation effects; Flavonoids; analysis; genetics; pharmacology; radiation effects; Free Radical Scavengers; pharmacology; Light; Medicine, Chinese Traditional; methods; Phytotherapy; methods; Plant Extracts; chemistry; Rehmannia; chemistry; Scutellaria baicalensis; chemistry; growth & development; Suspensions; chemistry
- From: China Journal of Chinese Materia Medica 2010;35(6):682-685
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of light on baicalin,baicalein accumulation and related genes expression in suspension cultures of Scutellaria baicalensis.
METHODThe content of baicalin, baicalein in suspension cultures of S. baicalensis was determined by HPLC. The related genes expression was analyzed by semi-quantitative PCR.
RESULTActive ingredients in suspension cultures of S. baicalensis were promoted significantly by light, PAL gene transcription under the conditions of light was higher than that under the dark conditions, the UBGAT gene expression levels was significantly related to the baicalein (r = -0.995).
CONCLUSIONActive ingredients and related genes (PAL, UBGAT) in suspension cultures of S. baicalensis were promoted significantly by light.
