Simultaneous determination of five active constitutents in Xiaochaihu Tang by HPLC.
- Author:
Qingchun LIU
1
;
Junning ZHAO
;
Liangchun YAN
;
Jinhai YI
;
Jun SONG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; Flavanones; analysis; Flavonoids; analysis; Glucosides; analysis; Glycyrrhizic Acid; analysis; Indicators and Reagents; analysis; Medicine, Chinese Traditional; Rehmannia; chemistry; Spectrophotometry, Ultraviolet; methods; Technology, Pharmaceutical
- From: China Journal of Chinese Materia Medica 2010;35(6):708-710
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a HPLC-PDA method for the determination of baicalin, wogonoside, baicalein, wogonin and glycyrrhizic acid in Xiaochaihu Tang.
METHODA Symmetry Shield RP18 (4.6 mm x 250 mm, 5.0 microm) was used with a mobile phase of acetonitrile-0.01% H3PO4 in gradient elution. The detection wavelength was 251 nm,the flow rate was 0.45 mL x min(-1) and the column temperature was maintained at 30 degrees C.
RESULTThe accuracy, precision, sensitivity, specificity and linearity of this method met the requirements. The contents of the five effective fractions were determined simultaneously.
CONCLUSIONThe method is rapid,simple and accurate and it can be suitable for the determination of baicalin, wogonoside, baicalein, wogonin and glycyrrhizic acid in Xiaochaihu Tang simultaneously.
