Simultaneous determination of atractylone, hinesol, beta-eudesmol, atrctylodin in Atractylodes lancea and hierarchical cluster analysis.
- Author:
Lei ZHANG
1
;
Zhen OUYANG
;
Ming ZHAO
;
Peixiang WANG
;
Jing FANG
Author Information
- Publication Type:Journal Article
- MeSH: Atractylodes; chemistry; Chromatography, High Pressure Liquid; methods; Cluster Analysis; Drugs, Chinese Herbal; analysis; Furans; analysis; Plant Extracts; chemistry; Plant Oils; analysis; Quality Control; Sesquiterpenes; analysis; Sesquiterpenes, Eudesmane; analysis; Spiro Compounds; analysis
- From: China Journal of Chinese Materia Medica 2010;35(6):725-728
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop a GC method for simultaneous determination of 4 compounds (atractylone, hinesol, beta-eudesmol and atractylodin) in Atractylodes lancea.
METHODA HP-1 capillary column (0.25 mm x 30 m, 0.25 microm) was used. The detector was FID:Inlet temperature was 250 degrees C. The detector temperature was 250 degrees C. The column temperature was set at 145 degrees C and held for 25 min after injection, then programmed at 10 degrees C x min(-1) to 250 degrees C and held for 10 min at the temperature. The carrying gas was nitrogen, split ratio was 40:1. Injection volume was 2 microL, Cluster analysis was performed by SPSS13.0 software.
RESULTThe linear ranges for atractylone, hinesol, beta-eudesmol and atractylodin were 0.0122. 32 (r = .9998), 0.008-1.68 (r = 0.9998), 0.009-1.76 (r = 0.9999), 0.016-3.20 g x L(-1) (r = 0.9997), respectively. The average recoveries (n = 3) of atractylone, hinesol, beta-eudesmol and atractylodin were 98.0%-99.0%, 97.7%-99.4%, 98.4%-99.2%, 97.8%-99.7%, respectively. The samples analyzed were divided into two classes.
CONCLUSIONThis method is simple, specific, repeatable and stable. It can be applied for the simultaneous determination of 4 compounds (atractylone, hinesol, beta-eudesmol and atractylodin) in A. lancea, which will provide the basis for the quality control of A. lancea. The contents of 4 active compounds were significantly different between geo-authentic and non-authentic producing areas.