Determination of kirenol in Herba Siegesbeckiae Preparata by high performance liquid chromatography.
- Author:
Zan LIU
1
;
Guixin CHOU
;
Zhengtao WANG
Author Information
- Publication Type:Journal Article
- MeSH: Acetonitriles; chemistry; Adenosine; analysis; Asteraceae; chemistry; Carcinogens; analysis; Chromatography, High Pressure Liquid; methods; Drugs, Chinese Herbal; chemistry; Quality Control; Sweetening Agents; analysis
- From: China Journal of Chinese Materia Medica 2010;35(6):729-731
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish a high performance liquid chromatographic method for the determination of kirenol in Herba Siegesbeckiae Praeparata.
METHODThe analysis was carried out on a Boston Crest ODS column (4.6 mm x 250 mm, 5 microm) with gradient elution using acetonitrile-water as mobile phases. The flow rate was 1.0 mL x min(-1) and the detection wavelength was at 215 nm.
RESULTThe calibration curve was linear over the range of 0.0202-20.02 microg for kirenol. The correlation coefficient of the calibration curve was 0.99975. The average recovery was 99.62% with relative standard derivation (RSD) of 2.0%.
CONCLUSIONThe results showed that the method is simple, accurate and repeatable and it is suitable for the determination of kirenol in Herba Siegesbeckiae Praeparata.
