Effect of Dureping injection on TIR signal pathway on Ana-1 cells.
- Author:
Yanli ZHANG
1
;
Ningping WANG
;
Ligang GU
;
Pengtao LI
Author Information
- Publication Type:Journal Article
- MeSH: Adaptor Proteins, Signal Transducing; Animals; Cells; Cells, Cultured; Epithelial Cells; drug effects; metabolism; Interleukin-1 Receptor-Associated Kinases; genetics; Macrophages; drug effects; metabolism; Mice; Myeloid Differentiation Factor 88; genetics; metabolism; NF-kappa B; metabolism; RNA, Messenger; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Signal Transduction; drug effects; TNF Receptor-Associated Factor 6; drug effects; genetics; metabolism; Transcription Factor RelA; metabolism
- From: China Journal of Chinese Materia Medica 2010;35(6):741-744
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the influence of Dureping injection to the murinal celiac macrophage Ana-1 on TIR signal pathway.
METHODAna-1 cell line was infected by influenza virus FM1 strain and treated with the Dureping injection in different concentrations (10.1 mg x L(-1) group) for 12 h and 24 h. Then we collected the cells, extracted mRNA and measured the expressions of TLR7, MyD88, IRAK4, TRAF6 and NF-kappaB p65 respectively by RT-PCR.
RESULTDureping injection down-regulated the expression of TLR7, MyD88, IRAK4, TRAF6 and NF-kappaB p65 mRNA in Ana-1 cell line infected by influenza virus, in a dose-dependent manner significantly.
CONCLUSIONDureping injection has an obvious effect against influenza virus FM1 strain by regulating the TIR signal pathway.
