Research on callus induction and plantlet regeneration of Prunella vulgaris.
- Author:
Wei SHENG
1
;
Li CHANG
;
Aimin ZHANG
;
Jianping XUE
;
Yuling LIU
Author Information
- Publication Type:Journal Article
- MeSH: Culture Media; chemistry; Plant Leaves; drug effects; physiology; Prunella; drug effects; physiology; Regeneration; drug effects; Time Factors
- From: China Journal of Chinese Materia Medica 2010;35(7):830-833
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the conditions of callus induction and plantlet regeneration of Prunella vulgaris.
METHODBy using the orthogonal experiment design, different explants, sucrose, plant growth substances and their ratio for callus induction and differentiation were optimized.
RESULT AND CONCLUSIONThe inductivity of leaves was the highest, followed by stems, but callus of the leafstalks could not be induced. The main factor of affecting the formation of callus was 6-BA. The optimal medium for callus induction was MS + 6-BA 3.0 mg x L(-1) + NAA 0.1 mg x L(-1) + 2,4-D 0.5 mg x L(-1) + sucrose 3%. The optimal medium for callus differentiation was 1/2 MS + 6-BA 3.25 mg x L(-1) + NAA 1.25 mg x L(-1) + sucrose 2%. The optimal medium for rooting was MS + IBA 1.0 mg x L(-1) + sucrose 3%.
