Determination of tractylodinol in different populations of Atractylodes lancea.
- Author:
Chengwu FANG
1
;
Min FAN
;
Shoujin LIU
;
Nianjun YU
Author Information
- Publication Type:Journal Article
- MeSH: Atractylodes; chemistry; Chromatography, High Pressure Liquid; Chromatography, Reverse-Phase; Linear Models; Organic Chemicals; analysis; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2010;35(7):834-836
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish an RP-HPLC method for determination of atractylodinol in Ateractylodes lancea and compare the contents of atractylodinol in the herbs of different origins.
METHODThe samples were separated on an Agilent TC-C18 (4.6 mm x 250 mm, 5 microm) column with the mobile phase of acetonitrile-water (49:51). Flow rate was 1.0 mL x min(-1). The detection wave length was set at 337 nm. Column temperature was 30 degrees C.
RESULTThe linear range of atractylodinol was 9.12 x 10(-2) -9.12 mg x L(-1) (r = 0.999 9), the average recovery was 97.15%, RSD was 1.5% (n = 5). The contents of atractylodinol were in the range of 0.268-1.213 mg x g(-1) in the samples from different orgins. The contents of atractylodinol in samples growing in Dabieshan mountain were higher than those in Jiangsu province (P < 0.001).
CONCLUSIONThe established method for determination of atractylodinol is accurate and reliable, which can be used to evaluate the quality of A. lancea, the contents of atractylodinol in the sample was related with its morphological characteristic and geographic orgin.
