Study on preparation of icaritin by enzymolysis of icariin with snail hydrolase.
- Author:
Dongsheng JIA
1
;
Xiaobin JIA
;
Jing XUE
;
Feng SHI
;
E SUN
;
Lingling CHEN
;
Zhenhai ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Flavonoids; metabolism; Hydrogen-Ion Concentration; Hydrolases; metabolism; Hydrolysis; drug effects; Kinetics; Metals; pharmacology; Snails; enzymology; Temperature
- From: China Journal of Chinese Materia Medica 2010;35(7):857-860
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo optimize the conditions for enzymolysis of Icariin by snail hydrolase.
METHODTake conversion rate as index, the effects of pH, temperature, reaction time, dosage of enzyme, concentration of Icariin and metal ion on hydrolysis were studied by single-factor designs and a L9 (3(4)) orthogonal design. The product was characterized through MS, 1H-NMR and 13C-NMR methods.
RESULTThe optimum enzymolysis conversion rate was achieved at 37 degrees C, pH 6.0 acetic acid-sodium acetate buffer solution and 48 h. The Ca2+, Mg2+ and Zn2+ had activation on helicase slightly but the Fe2+ inhibited enzymolysis significantly. The production was presumed as icaritin on the basis of spectral evidences.
CONCLUSIONSnail hydrolase can be used for preparing of icaritin. The condition is mildness and suitable for industrialization.
