Determination of tomatine in Solanum cathayanum by RP-HPLC.
- Author:
Jianghong GUO
1
;
Yuan ZHOU
;
Rong LIU
;
Mingxia XIE
;
Kun ZOU
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; methods; Chromatography, Reverse-Phase; methods; Solanum; chemistry; Tomatine; analysis
- From: China Journal of Chinese Materia Medica 2010;35(7):882-884
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo develop an HPLC method for determination of tomatine in the dried Solanum cathayanum of China Hubei Enshi.
METHODThe analysis was performed on a YMC-Pack ODS-AA column (4. 6 mm x 150 mm, 5 microm) eluted with acetonitrile and water in gradient mode. The concentration of acetonitrile in the mobile phase changes from 20% to 100% within 60 minutes. The detection wavelength was set 203 nm. The flow rate was 1.0 mL x min(-1) and column temperature was set at 30 degrees C.
RESULTThe linear relationship of tomatine was determined within the range from 0.1-0.6 g x L(-1) (r = 0.999 7). The average recovery as 98.93% with RSD 1.2%.
CONCLUSIONA convenient and reliable method was developed to determine the content of tomatine in the dried S. cathayanum.
