HPLC determination of chikusetsusaponin IVa in Rhizoma Panacis Majoris from different producing areas.
- Author:
Xiaomei SONG
1
;
Lin LI
;
Guangming YANG
;
Baochang CAI
Author Information
1. Nanjing University of Traditional Chinese Medicine, Nanjing 210046, China.
- Publication Type:Journal Article
- MeSH:
Chromatography, High Pressure Liquid;
Linear Models;
Oleanolic Acid;
analogs & derivatives;
analysis;
isolation & purification;
Panax;
chemistry;
Reproducibility of Results;
Rhizome;
chemistry;
Saponins;
analysis;
isolation & purification
- From:
China Journal of Chinese Materia Medica
2010;35(7):885-887
- CountryChina
- Language:Chinese
-
Abstract:
To determinate the chikusetsusaponin IVa in Rhizoma Panacis Majoris from different producing areas. The HPLC separation was performed on a Inertsil ODS-sp column (4.6 mm x 150 mm, 5 microm). A mixture of acetonitrile and 0.2% phosphoric acid solution (35:65) as the mobile phase the column. Temperature was set in 30 degrees C. The flow rate was 1.0 mL x min(-1), and the wave length of the detector is 203 nm. The content of the chikusetsusaponin IVa in Rhizoma Panacis Majoris from Meixian, Shaanxi is the highest and the lowest is from Enshi, Hubei. There have most differerence among the content of the chikusetsusaponin IVa in Rhizoma Panacis Majoris from different producing areas.
