Determination of eight kinds of ginsenosides in Shizhu ginseng.
- Author:
Junjie GAO
1
;
Yanjun ZHAI
;
Rongxiang WANG
;
Guqiang WANG
;
Bing WANG
;
Hui ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Chromatography, High Pressure Liquid; Ginsenosides; analysis; Linear Models; Panax; chemistry; Reproducibility of Results
- From: China Journal of Chinese Materia Medica 2010;35(8):989-991
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo establish the method for determining simultaneously eight kinds of ginsenoside Rg1, Re, Rf, Rg2, Rb1, Rc, Rb3 and Rd and their contents in Shizhu ginseng.
METHODHPLC was carried out on Agilent TC-C18 (4.6 mm x 150 mm, 5 microm) with acetonitrile -0.05% phosphoric acid, gradient elution, the volume of flow 1 mL x min(-1), column temperature 30 degrees C and detection wavelength 203 nm.
RESULTThe contents of eight kinds of ginsenosides in Shizhu ginseng were 1.32, 2.08, 0.72, 0. 24, 2.12, 1.06, 0.35, 0.55 mg x g(-1), respectively.
CONCLUSIONThe contents of ginsenoside ingredients in Shizhu ginseng were determined in the first time. The established method can be used in determining the contents of multiple gensenosides in herb ginseng simultaneously.
