Construction of a red fluorescent shuttle vector controlled by recA operon promoter of Streptococcus mutans.
- Author:
Wen-ming HUANG
1
;
Yang-long XU
;
De-qin YANG
Author Information
- Publication Type:Journal Article
- MeSH: Escherichia coli; genetics; metabolism; Fluorescent Dyes; Genes, Essential; Genes, Reporter; Genetic Vectors; Luminescent Proteins; genetics; Operon; Plasmids; Promoter Regions, Genetic; Rec A Recombinases; genetics; metabolism; Recombinant Proteins; genetics; metabolism; Streptococcus mutans; genetics; Transformation, Bacterial
- From: Chinese Journal of Stomatology 2012;47(5):291-295
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo construct a red fluorescent shuttle vector controlled by recA operon promoter to transform Streptococcus mutans.
METHODSThe promoter of recA was amplified from Streptococcus mutans UA159, and connected to plasmid pDsRed2-N1 to construct pRred with a red fluorescent coding gene, which was then inserted into the shuttle vector pDL276 to construct pLRred.
RESULTSpLRred was successfully constructed, and Escherichia coli transformed with the pLRred plasmid could express reporter gene DsRed.
CONCLUSIONSThe recombination plasmid pLRred can be used in the further research of the expression of cariogenic virulence factor gene by Streptococcus mutans in biofilm.
