Hepatitis B virus P22e protein inhibits human hepatocellular carcinoma HepG2 cell apoptosis in vitro.
- Author:
Zhi-hong DIAO
1
;
Ming-xia ZHANG
;
You-fu ZHU
;
Jin-lin HOU
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Carcinoma, Hepatocellular; metabolism; Hep G2 Cells; Hepatitis B Core Antigens; metabolism; Humans; Transfection; Viral Core Proteins; metabolism
- From: Journal of Southern Medical University 2007;27(11):1649-1652
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of the hepatitis B virus (HBV) P22e protein on the apoptosis of human hepatocellular carcinoma HepG2 cells.
METHODSHepG2 cells were transfected with recombinant plasmid pEGFP-HBVP22e and exposed to Act-D and tumor necrosis factor alpha (TNFalpha) treatment to induce cell apoptosis. Flow cytometry was performed to determine the proportion of cells containing sub-G1 DNA to represent the number of apoptotic cells. Laser scanning confocal microscopy was used to observe the nuclear alterations in the apoptotic cells.
RESULTSHepG2EGFP-C2HBVP22e cell strain showed a much delayed apoptosis as well as obviously lowered apoptotic rate in comparison with the HepG2 strain (P<0.01).
CONCLUSIONThe introduction and expression of extraneous gene HBVP22e significantly inhibits the apoptosis of HepG2 cells.
