L-arginine reduces intestinal epithelial cell apoptosis in rats with severe abdominal infection.
- Author:
Cheng-tang WU
1
;
Yong-feng REN
;
Jin-feng LIU
;
Jun-hua ZHANG
;
Shang-tong LEI
Author Information
- Publication Type:Journal Article
- MeSH: Abdominal Cavity; Animals; Apoptosis; drug effects; Arginine; pharmacology; Cecum; injuries; Disease Models, Animal; Epithelial Cells; drug effects; Infection; drug therapy; pathology; Intestinal Mucosa; cytology; drug effects; Rats; Rats, Wistar
- From: Journal of Southern Medical University 2007;27(11):1728-1730
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo explore the effect of L-arginine (L-Arg) on intestinal mucosal cell apoptosis in rats with severe abdominal infection.
METHODSEighteen Wistar rats were randomized into 3 groups, namely the CLP group (n=6) in which the rats were subjected to cecal ligation plus puncture (CLP) to induce severe abdominal infection, L-Arg group (n=6) where the rats received 300 mg/kg peritoneal L-Arg injection following CLP establishment, and the control group (n=6) where the rats underwent ventrotomy only. Intestinal epithelial apoptotic cells were quantified in each group using TUNEL assay 24 h after the operation.
RESULTSCompared with the control group, the rats in CLP and L-Arg groups showed significantly increased number of apoptotic cells in the intestinal epithelium 24 h after the operation (P<0.001). The apoptotic index (AI) in the L-Arg group (18.1-/+2.2) was significantly lower than that in CLP group (20.8-/+2.3, P=0.038).
CONCLUSIONSevere abdominal infection results in increased apoptosis of the intestinal epithelial cells in rats, and L-Arg treatment may reduce the cell apoptosis.
