Melittin inhibits proliferation and induces apoptosis of malignant human glioma cells.
- Author:
Zhi-lin YANG
1
;
Yi-quan KE
;
Ru-xiang XU
;
Ping PENG
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; drug effects; Cell Line, Tumor; Cell Proliferation; drug effects; Glioma; metabolism; pathology; Humans; Melitten; pharmacology
- From: Journal of Southern Medical University 2007;27(11):1775-1777
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the anti-tumor effects of melittin against malignant human glioma cells in vitro.
METHODSTwo malignant human glioma cell lines (U87 and U251) were treated with melittin at various concentrations, and the cell growth inhibition and apoptosis were evaluated using MTT assay, flow cytometry and agarose gel electrophoresis.
RESULTSMelittin could obviously inhibit the proliferation of the two glioma cell lines (P<0.05). At the concentrations of 1, 10, 20, 40, 80, 160, 200 mg/L, melittin resulted in U87 cell apoptosis rates of 12.80%, 16.92%, 22.69%, 34.05%, 41.82%, 59.87%, and 80.25%, and in U251 cell apoptosis rate of 11.61%, 16.21%, 22.03%, 30.57%, 41.10%, 58.33%, and 79.12%, respectively, showing a dose-dependent effect in its action of inducing cell apoptosis.
CONCLUSIONMelittin inhibits the proliferation and induces apoptosis of malignant human glioma cell lines in vitro.
