Expression of proliferating cell nuclear antigen in severely damaged small intestinal mucosa due to high-dose 5-FU exposure.
- Author:
Yu-qi LUO
1
;
Cheng-tang WU
;
Ying WEN
;
Kang-xiong LIAO
;
Jun-hua ZHANG
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Antimetabolites, Antineoplastic; adverse effects; Fluorouracil; adverse effects; Intestinal Mucosa; drug effects; metabolism; pathology; Intestine, Small; drug effects; metabolism; pathology; Mice; Mice, Inbred C57BL; Proliferating Cell Nuclear Antigen; metabolism
- From: Journal of Southern Medical University 2007;27(12):1860-1862
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo detect the expression of proliferating cell nuclear antigen (PCNA) in severely damaged intestinal mucosa due to high-dose 5-FU exposure.
METHODSThirty-two adult C57BL/6J mice were subjected to daily intraperitoneal high-dose 5-FU injection at 150 mg/kg for 5 consecutive days, and on days 1, 3, and 5, the mice were sacrificed to obtain the small intestinal tissue for HE straining and immunohistochemistry for detecting PCNA expression. Another 8 mice with intraperitoneal PBS injection served as the control group.
RESULTSHigh-dose 5-FU exposure of the mice resulted in severe intestinal mucous damage, with complete destruction of the villi and crypts and significantly increased cells positive for PCNA expression (P<0.01).
CONCLUSIONHigh-dose 5-FU treatment can significantly increase the PCNA index, and the cells expressing PCNA can be closely associated with regeneration of the severely damaged mucosa due to the exposure.
