Rearrangement of immunoglobulin heavy chain variable region genes in human neonates.
- Author:
Xiao-jian MAO
1
;
Xin XIAO
;
Ai-hua XIONG
;
Xin-hua QIAN
Author Information
- Publication Type:Journal Article
- MeSH: Gene Rearrangement; Genes, Immunoglobulin Heavy Chain; Gestational Age; Humans; Immunoglobulin Heavy Chains; genetics; Immunoglobulin Variable Region; genetics; Infant, Newborn; Multigene Family; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA
- From: Journal of Southern Medical University 2007;27(12):1901-1904
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the rearrangement of immunoglobulin (Ig) heavy chain variable region (V(H)) genes in human neonates with different gestational ages (GA).
METHODSPeripheral blood from the neonates with GA of 27 weeks (4 cases), 28-32 weeks (9 cases), 33-36 weeks (12 cases), and 37-42 weeks (13 cases) was collected. RT-PCR was used to amplify the Ig V(H) gene, and the PCR products were separated by electrophoresis and analyzed using 6% denaturing PAGE gel.
RESULTSAll Ig V(H) family genes had several rearranged genes in each GA group, and the neonates with different GA showed no significant difference in the median molecular weight for each rearranged Ig V(H) family gene.
CONCLUSIONThe neonates with GA of 27-42 weeks exhibit diversity in Ig V(H) gene rearrangement, and for the same Ig V(H) family, the median length of the arranged Ig V(H) genes is independent of the gestational age.
