Effect of aldosterone on aquaporin and ionophorous protein expressions in guinea pig cochlea.
- Author:
Qi LI
1
;
De-Liang HUANG
Author Information
- Publication Type:Journal Article
- MeSH: Aldosterone; pharmacology; Animals; Aquaporin 1; metabolism; Cochlea; drug effects; metabolism; Epithelial Sodium Channels; metabolism; Guinea Pigs; Immunohistochemistry; RNA, Messenger; metabolism; Reverse Transcriptase Polymerase Chain Reaction; Sodium-Potassium-Exchanging ATPase; metabolism; Up-Regulation
- From: Journal of Southern Medical University 2007;27(12):1918-1920
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effect of aldosterone on aquaporin and ionophorous protein expressions in the cochlea of guinea pigs.
METHODSRT-PCR was used to detect the expression of Na-K ATPase and epithelial sodium channel (ENaC) mRNA in the cochlea of guinea pigs 6 h after intraperitoneal aldosterone injection. Immunohistochemistry was used to detect the expression of aquaporin 1(AQP1) in the cochlea 1 month after the injection.
RESULTSEarly after aldosterone injection, the expression level of Na-K ATPase beta(1) and beta(3) subunit mRNAs remained unchanged and level of ENaC alpha subunit mRNA was up-regulated in the cochlear lateral wall (P<0.05), and 1 month after the injection, the expression of AQP1 protein was down-regulated (P<0.05).
CONCLUSIONThe effect of aldosterone on the cochlea is mediated possibly through its action on the genome, and aldosterone may cause ion concentration alterations to induce endolymphatic hydrops.
