X-ray irradiation enhances tumor necrosis factor receptor p55 expression in human colorectal cancer cells and inhibits the release of its soluble form in vitro.
- Author:
Lei GAO
1
;
Lan BAI
;
Wei-min SHI
;
Qing-zhen NAN
;
Zhan-bin MAO
Author Information
- Publication Type:Journal Article
- MeSH: Apoptosis; Cell Line, Tumor; Colorectal Neoplasms; genetics; metabolism; Enzyme-Linked Immunosorbent Assay; Flow Cytometry; Gene Expression Regulation, Neoplastic; Humans; Immunohistochemistry; Receptors, Tumor Necrosis Factor, Type I; genetics; metabolism; Tumor Necrosis Factor Decoy Receptors; genetics; metabolism; X-Rays
- From: Journal of Southern Medical University 2007;27(12):1921-1923
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo investigate the effects of X ray on human colorectal cancer cells for their tumor necrosis factor receptor-p55 (TNFR-p55) expression and release of soluble soluble TNFR-p55 (sTNFR-p55) in vitro.
METHODSThe protein expression of TNFR-p55 in Lovo cells exposed to X-ray was detected using immunohistochemistry, and enzyme-linked immunosorbent assay was used to examine the levels of sTNFR-p55 in the supernatants of the cell culture. The cell apoptosis of the exposed cells was analyzed with flow cytometry, and the changes in cell morphology were observed microscopically.
RESULTSX-ray exposure of cells resulted in a strong increase in TNFR-p55 expression of (P<0.01) and LoVo cell apoptosis (P<0.05). The levels of sTNFR-p55 in the supernatant of cells with X-ray exposure was significantly lowered in comparison with the levels before exposure (P<0.01). Optical microscopy showed that the exposed LoVo cells shrank and became spherical with cytoplasmic condensation and nuclear pyknosis.
CONCLUSIONX-ray exposure can induce LoVo cell apoptosis by increasing TNFR-p55 expression on the cell membrane and inhibiting the release of sTNFR-p55 in the supernatants.
