Peripheral blood p53 single nucleotide polymorphism analysis for early diagnosis of colorectal carcinoma.
- Author:
Qing LIU
1
;
Zhuo-sheng LAI
;
Wei WNAG
;
Yan ZHANG
;
Miao ZHOU
Author Information
- Publication Type:Journal Article
- MeSH: Adult; Case-Control Studies; Colorectal Neoplasms; diagnosis; genetics; DNA Mutational Analysis; DNA, Neoplasm; genetics; Early Detection of Cancer; Female; Humans; Male; Mutation; Polymorphism, Single Nucleotide; Tumor Suppressor Protein p53; genetics
- From: Journal of Southern Medical University 2007;27(12):1939-1940
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo evaluate the role of p53 gene mutation in colorectal carcinoma and assess the value of peripheral blood p53 single nucleotide polymorphism (SNP) in early diagnosis of colorectal carcinoma.
METHODSNSP in axons 5-8 of p53 gene was detected using ligase detection reaction-polymerase chain reaction (LDR-PCR) in the peripheral blood of 100 patients with colorectal cancer and 100 healthy subjects.
RESULTSThe mutation rate of p53 gene was 24% (24/120) in colorectal carcinoma patients and 0% (0/120) in the healthy subjects (P<0.05).
CONCLUSIONp53 plays an important role in the carcinogenesis of colorectal carcinoma, and SNP analysis for p53 gene can be helpful in early diagnosis of colorectal carcinoma.
