Effects of amitrole on thyroid hormone-associated gene transcription in FRTL-5 cells.
- Author:
Hong-Mei PAN
1
;
Li-Shi ZHANG
;
De-Sheng WU
Author Information
- Publication Type:Journal Article
- MeSH: Amitrole; toxicity; Animals; Cells, Cultured; Enzyme Inhibitors; toxicity; Epithelial Cells; cytology; drug effects; metabolism; Nuclear Proteins; genetics; Rats; Rats, Inbred F344; Receptors, Thyrotropin; genetics; Reverse Transcriptase Polymerase Chain Reaction; Thyroglobulin; genetics; Thyroid Gland; cytology; Thyroid Nuclear Factor 1; Transcription Factors; genetics; Transcription, Genetic; drug effects
- From: Journal of Southern Medical University 2008;28(1):12-15
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo observe the effects of amitrole on the transcription of thyroglobulin (tg), thyroid peroxidase (tpo), Na(+)/I- symporter (nis), Na(+)/I- symporter (nis), thyroid-stimulating hormone receptor (tshr), thyroid transcription factor 1 (ttf-1) and paired-domain protein-8 (pax-8) genes in FRTL-5 cells and investigate the mechanism of amitrole for intervening in thyroid hormone activity.
METHODSFRTL-5 cells were treated with amitrole at 0.001, 0.01 and 0.1 mg/ml for 24 h, respectively, after which the cells were collected for extraction of the total RNA. RT-PCR was used to examine the effects of amitrole on the transcription of tg, tpo, nis, tshr, pax-8 and ttf-1 genes in FRTL-5 cells.
RESULTSAmitrole significantly induced tg gene transcription at all the doses, but produced no obvious effects on tpo and nis gene transcription. At the concentration of 0.1 mg/ml, amitrole significantly reduced pax-8 and tshr gene transcription but increased ttf-1 gene transcription.
CONCLUSIONThe effects of amitrole on thyroid hormone activity may be related with its actions on tg, ttf-1, tshr and pax-8 gene transcription.
