Effect of Penicillium marneffei on TLR-2, TLR-4, and Dectin-1 expression and TNF-alpha production in macrophage.
- Author:
Wen-Jie ZHAO
1
;
Li-Yan XI
;
Li MA
Author Information
- Publication Type:Journal Article
- MeSH: Animals; Cells, Cultured; Lectins, C-Type; Macrophages, Peritoneal; cytology; immunology; metabolism; Male; Membrane Proteins; biosynthesis; Mice; Mice, Inbred BALB C; Nerve Tissue Proteins; biosynthesis; Penicillium; immunology; Toll-Like Receptor 2; biosynthesis; Toll-Like Receptor 4; biosynthesis; Tumor Necrosis Factor-alpha; biosynthesis
- From: Journal of Southern Medical University 2008;28(1):37-40
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVETo study the effects of heat-killed Penicillium marneffei (PM) on the expressions of toll-like receptor-4 (TLR-4), toll-like receptor-2 (TLR-2) and dendritic cell associated C-type lectin-1 (Dectin-1)and the production of the proinflammatory cytokine tumor necrosis factor-alpha (TNF-alpha). in mouse peritoneal macrophages.
METHODSMouse peritoneal macrophages were cultured in the presence of heat-killed yeast-phase PM for 24 h, and the average fluorescence intensity of TLR-2, TLR-4, and Dectin-1 in the macrophages was detected using flow cytometry. Fluorescent staining of the macrophages was performed to observe the fluorescence of TLR-2, TLR-4, and Dectin-1 with confocal microscopy. TNF-alpha mRNA in the cell culture supernatant was measured with real-time PCR, and TNF-alpha protein detected using enzyme-linked immunosorbent assay (ELISA).
RESULTSThe average fluorescence intensity of TLR-2, TLR-4 and Dectin-1 in the macrophages was increased in response to a 24-h PM stimulation, and the stimulated macrophages produced large amounts of TNF-alpha.
CONCLUSIONPM up-regulates the expression of TLR-2, TLR-4 and Dectin-1 in mouse peritoneal macrophages, and their expressions are directly associated with macrophage activation.
