Mechanical Strain Regulates Osteoblast Proliferation Through Ca-CaMK-CREB Signal Pathway.
- Author:
Yong GUO
1
,
2
;
Qi LV
3
;
Xian-Qiong ZOU
4
;
Zhi-Xiong YAN
4
;
Yu-Xian YAN
1
;
Author Information
1. Depantment of Bioengineering, College of Biotechnology, Guilin Medical University, Guilin, Guangxi 541004, China
2. Institute of Medical Equipment, Academy of Military Medical Sciences, Tianjin 300161, China.
3. Experiment Management Center, Logistical College of People Armed Police Forces, Tianjin 300162, China.
4. Depantment of Bioengineering, College of Biotechnology, Guilin Medical University, Guilin, Guangxi 541004, China.
- Publication Type:Journal Article
- From:
Chinese Medical Sciences Journal
2016;31(2):100-106
- CountryChina
- Language:English
-
Abstract:
Objective To investigate the effects of mechanical strain on Ca-calmodulin dependent kinase (CaMK)-cAMP response element binding protein (CREB) signal pathway and proliferation of osteoblasts.Methods Using a four-point bending device, MC3T3-E1 cells were exposed to mechanical tensile strains of 2500 µs and 5000 µs at 0.5 Hz respectively. The intracellular free Ca([Ca]i) concentration and calmodulin activity were assayed by fluorospectrophotometry, CaMK II β, CREB, and phosphorylated (activated) CREB (p-CREB) were assessed by Western blot, and cells proliferation was assayed with MTT. Pretreatment with verapamil was carried out to block Cachannel, and inhibitor U73122 was used to inhibit phospholipase C (PLC).Results Mechanical strains of 2500 µs and 5000 µs for 1 to 10 minutes both increased [Ca]i level of the cells. The 2500 µs strain, a periodicity of 1 h/d for 3 days, activated calmodulin, elevated protein levels of CaMK II β and p-CREB, and promoted cells proliferation, which were attenuated by pretreatment of verapamil or U73122. The effects of 5000 µs strain on calmodulin, CaMK II β, p-CREB and proliferation were contrary to 2500 µs strain.Conclusion The mechanical strain regulates osteoblasts proliferation through Ca-CaMK-CREB signal pathway via Cachannel and PLC/IPtransduction cascades.
