Up-regulation of Let-7a Expression Induces Gastric Carcinoma Cell Apoptosis In Vitro.
- Author:
Yimin ZHU
1
;
Fuying XU
1
Author Information
1. Department of Surgery, College of Medicine and Health, Lishui University/Lishui People's Hospital, Lishui, Zhejiang 323000, China.
- Publication Type:Journal Article
- MeSH:
Apoptosis;
Cell Line, Tumor;
Gene Expression Regulation, Neoplastic;
Humans;
MicroRNAs;
biosynthesis;
genetics;
RNA, Neoplasm;
biosynthesis;
genetics;
Stomach Neoplasms;
genetics;
metabolism;
pathology;
Up-Regulation
- From:
Chinese Medical Sciences Journal
2017;32(1):44-47
- CountryChina
- Language:English
-
Abstract:
Objective To investigate effect of let-7a gene over-expression on apoptosis of gastric cancer cell lines SGC-7901.Methods The stable let-7a gene over-expressing SGC-7901 cells, SGC-7901/let-7a cells, were established using shRNA lentiviral vector methods. Real-time RT-PCR analysis was used to evaluate the expression level of let-7a mRNA. Cells apoptosis was assessed by flow cytometry.Results RT-PCR analysis revealed let-7a expression in SGC-7901/let-7a cells was significantly increased. Cellular apoptosis assay showed that over-expression of let-7a could increase apoptosis of SGC-7901 cells (P=0.002).Conclusion Up-regulating let-7a expression promoted apoptosis in SGC-7901 cells.
